After plasmid purification of the impurities may be present in the stain that I can see the end of the gel? I did an extraction phenol chloroform and ethanol precipitation (Started with the culture of E. coli contains my plasmid – was in YT broth). The picture of my gel shows a spot on the [...]

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9. Why no template DNA strands reanneal each other during the PCR annealing cycle? also, at what temperature is used to extend the primers? makes this point: the new target sequence or does it mean that the first (target complementary sequence oligonucleotide) extend? The primers used in excess so that it is much more likely [...]